Outcome and success rate of vaginal birth after cesarean deliveries

The aim is to define the variables associated with vaginal birth after cesarean section and to identify the possible factors for predicting its success. Prospective observational study on women with previous single cesarean section undergoing vaginal delivery. Two hundred and forty pregnant women, full term, singleton, vertex presentation, in labour with a history of one cesarean section were studied. Full history, abdominal and vaginal examination, abdominal ultrasonography, careful monitoring and continuous cardiotocography were undertaken. Fetal weight and Apgar scoring were recorded. The success rate of vaginal birth after cesarean section was significantly influenced by previous vaginal delivery, cervical dilatation at admission and fetal weight. Body mass index, age, interval from previous cesarean section, nationality, sex of the newborn, gestational age at delivery or the use of oxytocin during labour did not affect the success rate of vaginal birth after cesarean deliverles, Uterine rupture and/or dehiscence were not encountered. Post delivery haemoglobin concentrations and hematocrite values were significantly higher in cases delivered vaginally. Vaginal birth after cesarean section conducted in a well equipped hospital, with careful moni toring is safe and successful with no maternal or fetal morbidities. Previous vaginal deliveries and non macrosomic fetal weight are the main contributors for the success of vaginal birth after cesarean section

Laser-Raman spectroscopy: a novel approach for sensitive molecular characterization of the mycobacterium genomic DNAs

In the present work we give a detailed analysis on the key Raman markers of genomic DNA for four species of Mycobacterium namely: Mycobacterium tuberculosis, Mycobacterium bovis, Mycobacterium avium and Mycobacterium phlei. The fundamental groups of bands have done for the first time to these Mycobacterium genomic DNAs. Molecular Laser Raman Spectroscopy [MLRS]; a novel and highly sensitive method, was used for whole genome characterization and comparison of the four species under investigation. The Raman spectroscopic analysis showed, in the M. avium and M. bovis the DNA is more rich in A+T as compared with the others, while the M. phlei and M tuberculosis are more rich in G+C. Moreover, a detailed analysis of all the bands diagnostic of B-form backbone geometry; the phosphate groups, bands diagnostic of deoxynucleoside conformation, ring vibrations of the bases, carbonyl stretching modes of thymine, guanine and cytosine was given here for the four mycobacterial species DNAs. The present study describes the use of [MLRS], as a novel promising assay, for rapid and discriminative detection of a mycobacterial genome with no confusion with other bacteria, utilizing genomes of four different mycobacteria species. In addition, Molecular Polymorphic Laser Raman Spectroscopy fingerprints and / or sequences may serve as markers that provide a basis for virulence, phylogenetic and evolutionary studies

In-vivo genotoxicity of the synthetic pyrethroid pesticide "cypermethrin" in rat liver cells by comet assay

The comet assay [single-cell gel electrophoresis, "SCGE"] is a simple method for measuring deoxyribonucleic acid [DNA] strand breaks in eukaryotic cells. The assay has applications in testing different chemical and physical agents for genotoxicity and monitoring environmental contamination with genotoxins. The objective of the present study was to evaluate the genotoxic effects of the synthetic pyrethroid pesticide "cypermethrin ", which is widely used in Egypt in pest- control programs in agriculture and in public health as well. Male rats were sacrificed 1, 7 or 14 clays after administration of single oral dose 1/30, 1/10 or 1/5 LD50 of commercial formulation of cypermethrin. Single liver cell suspensions were prepared and Comet assay was performed. With the SCGE assay, a clear induction of DNA damage was observed. It is generally noticed that all pesticide treatments yielded statistically significant [p<0.0001] DNA damage. In conclusion, cypermethrin induced a clear significant positive dose-dependent increase in DNA damage in the rat liver cells exposed to cypermethrin as compared with controls. But the effects in the SCGE were generally decreased with time after treatments. The results of the present work suggested that comet assay might be a suitable and sensitive endpoint in genotoxicity evaluation of pesticides, but we confirm that various tests should be used for detecting the mutagenic activity of pesticides

role of distribution of malasezia furfur serovars, propionibacteria and micrococcaceae in the pathogenesis of tinea versicolor and seborrhoeic dermatitis

This study was designed to compare the distribution of M. Furfur serovars [A, B and C], Propionibacteria and Micrococcaceae on the chest, face, back and scalp of 40 patients with tinea versicolor [TV] and ten matched controls and 40 patients with seborrhoeic dermatitis [SD] and ten matched controls. The patients and controls were sampled from the mentioned sites and cultured aerobically for Micrococcaceae and M. furfur and anaerobically for Propionibacteria. Serotyping of M. Furfur was carried out to identify the three serovars A, B and C. There was no difference in either the Micrococcaceae or Propionibacteria results in patients groups compared with control skin. Regarding the results of M. furfur, serovar A predominated in the back lesions in both patients groups when compared with the control. Serovar A was also predominant in the face lesions but only in seborrhoeic dermatitis patients, serovar C predominated in all sites [in both patient groups] and serovar B predominated in all lesions of TV patients and in the back, face and neck in SD patients